PCR Cloning Protocols, moment version, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the latest methods for DNA cloning and mutagenesis. right here the researcher will locate with no trouble reproducible equipment for all of the significant points of PCR use, together with PCR optimization, desktop courses for PCR primer layout and research, and novel adaptations for cloning genes of distinct features or starting place, with emphasis on lengthy distance PCR and GC-rich template amplification. additionally integrated are either traditional and novel enzyme-free and restrict site-free methods to clone PCR items right into a diversity of vectors, in addition to state of the art protocols to facilitate DNA mutagenesis and recombination, and to clone the difficult uncharacterized DNA flanking a recognized DNA fragment.
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